AB0099 CLONORCHIS SINENSIS-EXCRETORY/SECRETORY PROTEIN AMELIORATES INFLAMMATION IN ANKYLOSING SPONDYLITIS

Background Ankylosing spondylitis (AS) is a sort of inflammatory arthritis that affects axial skeleton, peripheral joints, and certain extra-articular organs, including the eyes, skin, gut. Recently, many attempts have been made to use parasite administration (e.g., ingestion eggs nematode Trichuris suis) as new modality for treating disorders. Our group published Clonorchis sinensis protein attenuated inflammation in AS. Objectives Thus, this study aimed assess therapeutic potential sinensis-Excretory/Secretory (Cs-ESP) Methods Cytotoxicity Cs-ESP at different doses was assessed by MTS flow cytometry before performing experiments. Peripheral blood mononuclear cells (PBMCs) synovial fluid (SFMCs) were obtained from AS patients. Inflammatory cytokine-producing analyzed using cytometry. The levels INF-g, IL-17A, TNF-a, IL-6 measured enzyme-linked immunosorbent assay (ELISA). SKG mice treated with or vehicles. Inflammation evaluated immunohistochemistry. Results Treatment resulted no reduced cell viability PBMCs SFMCs. In experiments culturing SFMCs, frequencies IFN-g IL-17A producing significantly after treatment. mouse model, treatment suppressed enthesitis. Conclusion We provide evidence demonstrating can ameliorate clinical signs cytokine derangements Figure 1. ameliorates symptoms References [1]Kim TJ, Kim TH. Clinical spectrum ankylosing Korea. Joint Bone Spine (2010) 77(3):235–40. [2]Martinez FD. coming-of-age hygiene hypothesis. Respir Res (2001) 2(3):129–32. [3]Summers RW, Elliott DE, Urban JF Jr., Thompson RA, Weinstock JV. suis therapy active ulcerative colitis: randomized controlled trial. Gastroenterology (2005) 128(4):825–32. [4]Osada Y, Shimizu S, Kumagai T, Yamada Kanazawa T. Schistosoma mansoni infection reduces severity collagen-induced via downregulation pro-inflammatory mediators. Int J Parasitol (2009) 39(4):457– 64. [5]Song X, Shen J, Wen H, Zhong Z, Luo Q, Chu D, et al. Impact japonicum on DBA/1 mice: murine model human rheumatoid arthritis. PloS One (2011) 6(8):e23453. Acknowledgements: NIL. Disclosure Interests None Declared.